3'-Methyl-4- (dimethylamino) azobenzeneラット投与期間中におけるグルコース代謝のラジオレスピロメトリーによる解析

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タイトル別名
  • Radiorespirometric Analysis of Glucose Metabolism in the Rat during Feeding with 3'-Methyl-4- (dimethylamino) azobenzene
  • 3 Metoyl 4 dimethylamino azobenzene ラット
  • -Radiorespirometry using [1-<SUP>14</SUP>C] glucose-
  • ―〔1-<SUP>14</SUP>C〕glucoseを用いたラジオレスピロメトリー―

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We estimated the enzyme activities on the hexose monophosphate oxidative pathway of glucose metabolism at an early stage during feeding with 3'-methyl-4- (dimethylamino) azobenzene (3'-Me-DAB) in the first paper of this series. In the present paper, the importance of this non-glycolytic pathway of glucose metabolism in preneoplastic liver in azo-dye fed rat was studied by radiorespirometry using [1-14C] glucose and [6-14C] glucoce. In addition to radioisotope analysis, the level of activity of glucose-6-phosphate dehydrogenase was measured in rat liver after various periods of feeding with 3'-Me-DAB.<BR>In radiorespirometry using [1-14C] glucose, the peak time was delayed up to the 2nd week, but was early by the 4th week and was the same as the control at the 5th week and thereafter. On the other hand, the peak height and the yield value both changed in almost the same way. That is to say, they were lower than those of the control until the 2nd week, began to increase from the 3rd week, reached a maximum at the 4th week, and remained at the control level at the 5th week and thereafter. Moreover, the yield value ratio of respiratory 14CO2 from [1-14C] glucose to that from [6-14C] glucose increased from 1.3 to 1.52 at the 3rd to 4th week after feeding with 3'-Me-DAB.<BR>The activity of glucose-6-phosphate dehydrogenase increased as early as the 2nd week after the start of feeding with 3'-Me-DAB, reaching a maximum at the 4th week. Thereafter, the activity remained high with a slight decrease from the maximum by the 6th week.<BR>The incorporations of [3H] thymidine and [U-14C] glucose into rat liver DNA at the 4th week after feeding with 3'-Me-DAB were both as high as 2 to 4 times those of the control.

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