Xray Irradiation Promotes Nerve Growth Factor-induced Neurite Extension in PC12 Cells

Katoh Shinsuke, Kobayashi Junya, Umeda Tomonobu, Kobayashi Yoshiko, Izumo Nobuo, Suzuki Takahiko

doi:10.3769/radioisotopes.65.137

Nerve growth factor (NGF) induces neurite extension in PC12 cells, a well-known model of neuronal differentiation. Previous reports have shown that irradiation activates the extracellular signal-regulated kinase 1/2 (ERK), which is a mainstream pathway for NGF signaling. This suggests that the neurite extension may possibly be enhanced by Xray irradiation. Therefore, we observed NGF-induced neurite extension with Xray irradiation in PC12 cells. At the start of the NGF treatment, Xray irradiation was performed simultaneously for the first 5 min. After 5 days, the irradiation at 500 mGy promoted NGF-induced neurite extension, and increased phosphorylation of ERK, but not of the NGF receptor. The phosphorylation of epidermal growth factor (EGF) receptor, a stimulatory growth signal in PC12 cells, was also enhanced by irradiation. Interestingly, AG1478, an inhibitor of EGF receptor tyrosine kinase, inhibited the irradiation-induced promotion of neurite extension. These results suggest that irradiation activate EGF receptor tyrosine kinase and promote NGF-induced neurite extension via the cross talk between NGF and EGF signaling.

Xray Irradiation Promotes Nerve Growth Factor-induced Neurite Extension in PC12 Cells

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