Study of Subcellular Distribution of <SUP>67</SUP>Ga in Tumor and Liver

ANDO Atsushi, TAKESHITA Masazumi, ANDO Itsuko, HIRAKI Tatsunosuke, HISADA Kinichi

doi:10.3769/radioisotopes.26.2_86

Subcellular distribution of⁶⁷Ga was quantitatively determined to evaluate the role of lysosome in accumulation of ⁶⁷Ga in malignant tumor tissue and liver. The following animals and transplanted tumors were used: rats implanted with Yoshida sarcoma and hepatoma AH109A; mice implanted with Ehrlich tumor. ⁶⁷Ga-citrate were injected to the rats intravenously and to the mice intraperitoneally. Ten minutes to 48 hours after the administration of ⁶⁷Ga-citrate, the animal were sacrificed, and the tumor tissues and liver were excised. Subcellular fractionation of tumor tissues and livers were carried out according to the method of Hogeboom and Schneider. Radioactivity of each fraction was counted by a well type scintillation counter, and protein of each fraction was measured according to Lowry's method.<BR>In Yoshida sarcoma and Ehrlich tumor, most of the radioactivity was localized in the supernatant fraction, and small amount of radioactivity was localized in the mitochondrial fraction (lysosome contains in this fraction) . But in the liver, most of the radioactivity was concentrated in the mitochondrial fraction and the radioactivity of this fraction was increased with the passage of time after administration. Twenty-four hours later, about 50% of total radioactivity was accumulated in this fraction. In the case of hepatoma AH109A, radioactivity of mitochondrial fraction was increased with the passage of time after administration, and about 30% of total activity was concentrated in this fraction at 24 hours after administration. From these results it is concluded that lysosome does not play an important role in the tumor concentration of ⁶⁷Ga and lysosome plays an important role in the liver concentration of ⁶⁷Ga. In the case of hepatoma AH109A it is presumed that lysosome plays considerably important role in the tumor concentration of ⁶⁷Ga, hepatoma AH109A having some nature of liver.

Study of Subcellular Distribution of <SUP>67</SUP>Ga in Tumor and Liver

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