Discrimination between endotoxin and (1-3)-beta-D-glucan using turbidimetric kinetic assay with Limulus amebocyte lysate.
書誌事項
- タイトル別名
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- Discrimination between endotoxin and (1.RAR.3)-.BETA.-D-glucan using turbidimetric kinetic assay with Limulus amebocyte lysate.
- Discrimination between endotoxin and (1→3)-β-d-glucan using turbidimetric kinetic assay with Limulus amebocyte lysate
抄録
A procedure for the Limulus amebocyte lysate (LAL) test discriminating between endotoxin and (1→3)-β-D-glucan based on the turbidimetric kinetic method was proposed. Endotoxin and (1→3)-β-D-glucan, which are elicitors of the activation of LAL, showed different reaction courses with this lysate. To analyze the difference in the reaction, two parameters, the maximum differential coefficient of the reaction (Dmax) and the reaction time required to botain Dmax (Tp) were defined. The logarithmic plottings of Tp versus Dmax (Tp-Dmax plot) discriminated between endotoxin and (1→3)-β-D-glucan. Endotoxin was measured with a standard curve plotting logarithmic endotoxin concentration versus Dmax (ET-Dmax plot). The endotoxin calculated from Dmax was less influenced by (1→3)-β-D-glucan than that calculated from the usual gelation time. A small amount of endotoxin in a sample could be concealed by the addition of polymyxin B, which inhibited the activation of LAL by endotoxin. (1→3)-β-D-glucan was measured without being affected by the presence of a small amount of endotoxin using LAL with polymyxin B. The following procedure is proposed as a LAL test to discriminate between endotoxin and (1→3)-β-D-glucan.(1) Identify the main substance (endotoxin or (1→3)-β-D-glucan) triggering the activation of LAL using the Tp-Dmax plot. (2) Use the appropriate method to measure the main substance : the ET-Dmax plot for endotoxin or the LAL with polymyxin B for (1→3)-β-D-glucan.
収録刊行物
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- ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ
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ＣＨＥＭＩＣＡＬ ＆ ＰＨＡＲＭＡＣＥＵＴＩＣＡＬ ＢＵＬＬＥＴＩＮ 38 (9), 2523-2526, 1990
公益社団法人 日本薬学会