Reverse-phase HPLC Separation of D-Amygdalin and Neoamygdalin and Optimum Conditions for Inhibition of Racemization of Amygdalin.

DOI Web Site Web Site PubMed 16 References
  • Hwang Eun-Young
    Department of Oriental Pharmaceutical Sciences, Kyung Hee University
  • Lee Je-Hyun
    Department of Oriental Pharmaceutical Sciences, Kyung Hee University
  • Lee Yong-Moon
    College of Pharmacy, Chungbuk National University
  • Hong Seon-Pyo
    Department of Oriental Pharmaceutical Sciences, Kyung Hee University

Search this article

Description

In boiling aqueous solution, D-amygdalin usually begins to convert into neoamygdalin in 3 min and more than 30% of the initial D-amygdalin is found as neoamygdalin after 30 min. In this report, we establish methods for simple HPLC analysis and the inhibition of D-amygdalin conversion. D-Amygdalin and its conversion product, neoamygdalin, were clearly separated on reverse-phase column chromatography by an optimized eluent of 10 mM sodium phosphate buffer (pH 3.8) containing 6% acetonitrile. Linearity for analyzing D-amygdalin and neoamygdalin was observed in the range from 0.05 to 0.5 mM. The detection limits for D-amygdalin and neoamygdalin were ca. 5 μM per injected amount. We found that D-amygdalin conversion was completely inhibited by adding 0.05% citric acid to the aqueous solution before boiling. To prevent the loss of pharmaceutical potency of Tonin, we applied this method to measure the conversion rate of D-amygdalin. We confirmed that D-amygdalin conversion in Tonin is effectively inhibited by acidic boiling solution with 0.1% citric acid.

Journal

References(16)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top