Purification and Characterization of Ginsenoside-β-Glucosidase from Ginseng
-
- ZHANG Chunzhi
- Department of Biotechnology, Dalian University of Technology
-
- YU Hongshan
- Department of Food Science & Biotechnology, Dalian Institute of Light Industry
-
- BAO Yongming
- Department of Biotechnology, Dalian University of Technology
-
- AN Lijia
- Department of Biotechnology, Dalian University of Technology
-
- JIN Fengxie
- Department of Food Science & Biotechnology, Dalian Institute of Light Industry
書誌事項
- タイトル別名
-
- Purification and Characterization of Ginsenoside-.BETA.-Glucosidase from Ginseng.
- Purification and Characterization of Ginsenoside ベータ Glucosidase from Ginseng
この論文をさがす
抄録
The ginsenoside-β-glucosidase that hydrolyzes the β-(1→2)-glucoside of the ginsenoside Rg3 sugar moiety to ginsenoside Rh2 was isolated from the ginseng root, and the enzyme was purified and characterized. The enzyme was purified to one spot in SDS polyacrylamide gel electrophoresis, and its molecular weight was about 59 kDa. The optimum temperature of the ginsenoside-β-glucosidase was 60 °C, and the optimum pH was 5.0. Ca2+ ion had positive effect on ginsenoside-β-glucosidase, while Cu2+ had negative effect on it. The ginsenoside-β-glucosidase may be a special β-glucosidase that is different from the original exocellulase such as β-glucosidase (EC 3.2.1.21).
収録刊行物
-
- CHEMICAL & PHARMACEUTICAL BULLETIN
-
CHEMICAL & PHARMACEUTICAL BULLETIN 49 (7), 795-798, 2001
公益社団法人 日本薬学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282679140546432
-
- NII論文ID
- 110003615996
- 30023110690
-
- NII書誌ID
- AA00602100
-
- COI
- 1:CAS:528:DC%2BD3MXkvVart7Y%3D
-
- ISSN
- 13475223
- 00092363
-
- NDL書誌ID
- 5820200
-
- PubMed
- 11456082
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可