Purification and Characterization of Ginsenoside-.BETA.-Glucosidase from Ginseng.
-
- ZHANG Chunzhi
- Department of Biotechnology, Dalian University of Technology
-
- YU Hongshan
- Department of Food Science & Biotechnology, Dalian Institute of Light Industry
-
- BAO Yongming
- Department of Biotechnology, Dalian University of Technology
-
- AN Lijia
- Department of Biotechnology, Dalian University of Technology
-
- JIN Fengxie
- Department of Food Science & Biotechnology, Dalian Institute of Light Industry
Bibliographic Information
- Other Title
-
- Purification and Characterization of Ginsenoside-β-Glucosidase from Ginseng
- Purification and Characterization of Ginsenoside ベータ Glucosidase from Ginseng
Search this article
Abstract
The ginsenoside-β-glucosidase that hydrolyzes the β-(1→2)-glucoside of the ginsenoside Rg3 sugar moiety to ginsenoside Rh2 was isolated from the ginseng root, and the enzyme was purified and characterized. The enzyme was purified to one spot in SDS polyacrylamide gel electrophoresis, and its molecular weight was about 59 kDa. The optimum temperature of the ginsenoside-β-glucosidase was 60 °C, and the optimum pH was 5.0. Ca2+ ion had positive effect on ginsenoside-β-glucosidase, while Cu2+ had negative effect on it. The ginsenoside-β-glucosidase may be a special β-glucosidase that is different from the original exocellulase such as β-glucosidase (EC 3.2.1.21).
Journal
-
- Chemical and Pharmaceutical Bulletin
-
Chemical and Pharmaceutical Bulletin 49 (7), 795-798, 2001
The Pharmaceutical Society of Japan
- Tweet
Details 詳細情報について
-
- CRID
- 1390282679140546432
-
- NII Article ID
- 110003615996
- 30023110690
-
- NII Book ID
- AA00602100
-
- COI
- 1:CAS:528:DC%2BD3MXkvVart7Y%3D
-
- ISSN
- 13475223
- 00092363
-
- NDL BIB ID
- 5820200
-
- PubMed
- 11456082
-
- Text Lang
- en
-
- Data Source
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- Abstract License Flag
- Disallowed