Effect of Polymethylene and Phenylene Linking Groups on the DNA Cleavage Specificity of Distamycin-Linked Hydroxamic Acid-Vanadyl Complexes.
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Abstract
Two types of distamycin-linked hydroxamic acids (DHA), which contain various lengths of polymethylene chains (PM-DHA) and relatively rigid phenylene ones (Ph-DHA), have been synthesized for the first time. Their DNA cleavage specificities were investigated by an end-labeled fragment cleavage experiment in the presence of vanadyl ion and hydrogen peroxide. The DNA cleavage by the PM-DHA·VO(II) complexes was shown to be very dependent on the length of the chain and the AT sequences. The tetramethylene DHA (1b) complex exhibited highly specific cleavage patterns flanking the 8 and 10 AT sites. Interestingly, the Ph-DHA complexes selectively cleaved the 5' end-labeled strand at the AT sites, but did not cleave the 3' end-labeled strand. The vanadyl complexing moieties and the local sequence conformation of the AT tract are suggested to contribute significantly to the DNA recognition of the PM-DHA·VO(II) complexes.
Journal
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- Chemical and Pharmaceutical Bulletin
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Chemical and Pharmaceutical Bulletin 48 (5), 603-609, 2000
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390282679141484800
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- NII Article ID
- 130003774082
- 110003635518
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- NII Book ID
- AA00602100
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- COI
- 1:CAS:528:DC%2BD3cXjtFWhtrg%3D
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- ISSN
- 13475223
- 00092363
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- NDL BIB ID
- 5364789
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- PubMed
- 10823693
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed