Reversible effect of calcium-binding protein on the Ca2+-induced activation of succinate dehydrogenase in rat liver mitochondoria.

YAMAGUCHI MASAYOSHI, SHIBANO HIROSUKE

doi:10.1248/cpb.35.3766

The effect of calcium-binding protein (CaBP) isolated from rat liver cytosol on the Ca²⁺-induced increase in succinate dehydrogenase activity of rat liver mitochondria was investigated. The liver mitochondrial succinate dehydrogenase activity was significantly increased by addition of Ca²⁺ in the range of 1.0-50 μM. Of various other metals (25 μM) tested, addition of Hg²⁺, Zn²⁺ and Cd2 + caused a significant decrease in succinate dehydrogenase activity of the hepatic mitochondria, while Mg²⁺, Mn²⁺ and Sr²⁺ had no effect;the increase in the enzyme activity was specific for Ca²⁺. This Ca²⁺-induced increase in the mitochondrial succinate dehydrogenase activity was completely blocked by the presence of ruthenium red (10 and 50 μM), an inhibitor of mitochondrial Ca²⁺ uptake, indicating that Ca²⁺ was transported into the mitochondria and activated the enzyme there. In the presence of more than 7.0 μM CaBP, the Ca²⁺ (10 μM) -induced increase in succinate dehydrogenase activity was completely reversed, but 1.4 μM CaBP had no effect on this increase. CaBP itself (15 μM) did not have an inhibitory effect on the basal activity of succinate dehydrogenase. The present results suggest that CaBP regulates the increase in succinate dehydrogenase activity by blocking Ca²⁺ transport into the hepatic mitochondria of rats.

Reversible effect of calcium-binding protein on the Ca2+-induced activation of succinate dehydrogenase in rat liver mitochondoria.

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