Separatory Determination of Glucuronic Acid and Glucuronides in Biological Fluid by Ion-Exchange Resin.

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公開日
1959
DOI
  • 10.1248/cpb.7.685
公開者
公益社団法人 日本薬学会

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説明

For the separatory determination of free glucuronic acid and conjugated glucuronic acids of various types, such as N-glucuronides and ester-type or ether-type O-glucuronides in urine, a revised method using a column of anion-exchange resin was investigated. A column of Amberlite IRA-411, after passing a mixed solution of sodium glucuronate, sodium aniline or 2-naphthylamine glucosiduronate, and phenyl glucosiduronic acid or potassium menthyl O-glucosiduronate, was first eluted with ammonium chloride solution at pH 8 to separate the free glucuronic acid, and then eluted with hydrochloric acid to hydrolyse and remove the N-glucuronide. The column was further eluted with methanolic hydrochloric acid to remove the stable O-glucuronide. For the colorimetric measurement of glucuronic acid in each of eluted fractions, a modified procedure of Dische's method using carbazole was employed, in which carbazole was added with sulfuric acid to the sample in order to save the time required for the whole procedure. By this improved method, so far as the present samples were concerned, the separatory and recovery determination of glucuronic acid, N-glucuronide, and O-glucuronide, excluding ester-type O-glucuronide, in urine, was quite satisfactory.

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