Chitosan Nanoparticles of 5-Fluorouracil for Ophthalmic Delivery: Characterization, in-Vitro and in-Vivo Study
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- Nagarwal Ramesh Chand
- Department of Pharmaceutics, Institute of Technology, Banaras Hindu University
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- Nath Singh Paras
- Department of Pharmaceutics, Institute of Technology, Banaras Hindu University
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- Kant Shri
- Department of Ophthalmology, Institute of Medical Sciences, Banaras Hindu University
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- Maiti Pralay
- School of Material Science & Technology, Institute of Technology, Banaras Hindu University
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- Pandit Jayanta Kumar
- Department of Pharmaceutics, Institute of Technology, Banaras Hindu University
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The aim of this investigation was to develop 5-fluorouracil (5-FU) loaded chitosan nanoparticles (CH-DNPs) for ophthalmic delivery. CH-DNPs were fabricated by ionotropic gelation mechanism using chitosan (CH) and a polyanion (TPP). The nanoparticles were smooth and spherical, confirmed by scanning electron microscopy (SEM) and atomic force microscope (AFM). CH/TPP mass ratio and TPP significantly changed the particles size morphology and encapsulation efficiency. The nanoparticles size ranged from approximately 114 to 192 nm and had a positive zeta potential (30±4 mV). The encapsulation efficiency, loading capacity and recovery of DNPs were 8.12—34.32%, 3.14—15.24% and 24.22 to 67% respectively. Physical characterization was done by Fourier transform infrared (FT-IR) and X-ray diffraction (XRD). No interaction was observed in between drug and polymer and crystallinity of drug was not changed in drug loaded nanoparticles. In-vitro release study of DNPs showed diffusion controlled release. Bioavailability study of batch CS9 was studied in rabbit eye and compare to 5-FU solution. 5-FU level was significantly higher in aqueous humor of rabbit eye. Ocular tolerance was studied in the eye of New Zealand rabbits and tested formulation was non-irritant with no sign of inflammation.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 59 (2), 272-278, 2011
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679149814912
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- NII論文ID
- 130000405527
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 10951944
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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