Comparative Study of Puerariae lobatae and Puerariae thomsonii by HPLC-Diode Array Detection-Flow Injection-Chemiluminescence Coupled with HPLC-Electrospray Ionization-MS

DOI Web Site Web Site 参考文献25件
  • Zhang Cui-Ling
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University Department of Traditional Chinese Medicine, Beng Bu Medical College
  • Ding Xiao-Ping
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University
  • Hu Zheng-Fang
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University
  • Wang Xin-Tang
    Department of Pharmacognosy, China Pharmaceutical University
  • Chen Lin-Lin
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University
  • Qi Jin
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University
  • Yu Bo-Yang
    Department of Complex Prescription of Traditional Chinese Medicine, China Pharmaceutical University

この論文をさがす

抄録

An on-line HPLC-diode array detection-flow injection chemiluminescence (HPLC-DAD-FICL) method was applied to estimate the difference of Puerariae lobatae and Puerariae thomsonii. Their chemical and active profiles could be obtained by HPLC-DAD-FICL in one run. Seventeen compounds in two species were tentatively identified by HPLC-electrospray ionization-MS (HPLC-ESI-MS) method. The main antioxidants were rapidly screened by active fingerprints coupled with MS data. Similarity and Hierarchical clustering analysis (HCA) were used to distinguish different samples. The results suggested that the chemical fingerprints of 16 batches of samples were similar by similarity evaluation, while HCA could discriminate the two species. The active fingerprints of Puerariae lobatae and Puerariae thomsonii were significantly different. More antioxidants were found in Puerariae lobatae than in Puerariae thomsonii. Main antioxidants, including 3′-hydroxypuerarin, genistein 8-C-glycoside-xyloside, puerarin, 6″-O-xylosylpuerarin, mirificin and daidzein in two species, may be reasonable markers for the discrimination of the two species. The integrated fingerprint based on the chemical and active characteristics may provide an objective quality evaluation for Puerariae lobatae and Puerariae thomsonii.

収録刊行物

参考文献 (25)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ