Simultaneous Voltammetric Determination of Ascorbic Acid, Dopamine and Uric Acid Using Polybromothymol Blue Film-Modified Glassy Carbon Electrode

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  • Xu Xiongwei
    Department of Pharmacy, First Affiliated Hospital of Fujian Medical University Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University
  • Lin Qihuang
    Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University
  • Liu Ailin
    Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University
  • Chen Wei
    Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University
  • Weng Xiuhua
    Department of Pharmacy, First Affiliated Hospital of Fujian Medical University
  • Wang Changlian
    Department of Pharmacy, First Affiliated Hospital of Fujian Medical University
  • Lin Xinhua
    Department of Pharmaceutical Analysis, Faculty of Pharmacy, Fujian Medical University

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A sensitive and selective electrochemical method for simultaneous determination of ascorbic acid (AA), dopamine (DA), and uric acid (UA) using an electropolymerized bromothymol blue (BTB)-modified glassy carbon electrode (GCE) was developed. The electrochemically synthesized film was investigated using electrochemical impedance spectroscopy and voltammetric methods. The electrochemical behavior of the polymer-modified electrode depends on film thickness, i.e., the electropolymyerization time. The poly-BTB-modified GCE shows excellent electrocatalytic activity toward the oxidation of AA, DA, and UA in phosphate buffer solution (pH 5.0). The voltametric peak separations of AA/DA, DA/UA, and AA/UA on this modified electrode are 118 mV, 298 mV, and 455 mV, respectively. Therefore the voltammetric responses of these three compounds can be resolved well on the polymer-modified electrode, and simultaneous determination of these three compounds can be achieved. In addition, this modified electrode can be successfully applied to determine AA and DA in injection and UA in urine samples without interference.

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