Studies on the Metabolism of Sulfadiazine. II. Quantitative Separation of Excrements in the Human Urine after Administration of Sulfadiazine
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- Other Title
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- Studies on the metabolism of sulfadiazine.-2-Quantitative separation of excrements in the human urine after administration of sulfadiazine
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Abstract
Excretion of sulfadiazine, N4-acetylsulfadiazine, sulfadiazine-N4-glucuronide, sulfadiazine-N4-sulfonate, and sulfanilamide into human urine was separately determined by paper chromatography by the following method. One tenth ml. of urine obtained after oral administration of sulfadiazine was spotted lineary on a filter paper (Toyo Roshi No.51 40×40 cm.) and developed ascendingly with a solvent system of butanol saturated with water and a solvent of BuOH-AcOh-H2O(5 : 1 : 4) for about 20 hours. The paper was dried in air, portions corresponding to the products were cut out, and eluted descendingly with N/4 ammonium hydroxide. Each of these metabolites was hydrolyzed with hydrochloric acid and determined by the colorimetric method using the Ehrlich's reagent as sulfadiazine. The content was calculated from a calibration curve prepared beforehand. The amount excreted 48 hours after oral administration of 2 g. of sulfadiazine was 1620 mg., calculated as sulfadiazine, including 862 mg. of sulfadiazine, 730 mg.of N4-acetylsulfadiazine, 11 mg. of sulfadiazine-N4-glucuronide, 14 mg. of sulfadiazine-N4-sulfonate, and 5 mg. of sulfanilamide, calculated as sulfadiazine respectively.
Journal
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- Chemical and Pharmaceutical Bulletin
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Chemical and Pharmaceutical Bulletin 14 (7), 687-691, 1966
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679151087360
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- NII Article ID
- 110003619920
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- NII Book ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL BIB ID
- 8508744
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed