Synthesis of 1-(4-Aminobutyl)-2-iminoimidazolidin-4-one Aimed at Preparation of a Creatinine-Specific Antibody

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  • Moriya Shun-suke
    Translational Medical Research Center, Tokyo Metropolitan Institute of Medical Science
  • Terayama Naoki
    Department of Applied Chemistry, Kogakuin University
  • Hiramatsu Kyoko
    Translational Medical Research Center, Tokyo Metropolitan Institute of Medical Science
  • Kawakita Masao
    Translational Medical Research Center, Tokyo Metropolitan Institute of Medical Science
  • Kiryu Tatsuya
    Department of Applied Chemistry, Kogakuin University
  • Kawanishi Takato
    Department of Applied Chemistry, Kogakuin University
  • Yasui Eiko
    Department of Applied Chemistry, Kogakuin University
  • Nagumo Shinji
    Department of Applied Chemistry, Kogakuin University

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For the purpose of obtaining a creatinine-specific antibody, a creatinine derivative with 4-aminobutyl, which was served as a linker for preparing the creatinine–bovine serum albumin (BSA) conjugate, was synthesized from 4-benzylaminobutan-1-ol in 8 steps. Production of anti-creatinine antibodies was observed in two rabbits using the creatinine–BSA conjugate, although their titer was rather low.

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