Studies on fungal polysaccharides,pt.1

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  • Studies on Fungal Polysaccharides. I. On the Isolation and Chemical Properties of Capsular Polysaccharide from Cryptococcus neoformans.

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Capsular polysaccharide of Cryptococcus neoformans CRD-1 was isolated by Einbinder's method and purified by zone electrophoresis with starch gel and borate buffer (pH 10.0) or acetate buffer (pH 5.0). This material (potassium salt) showed physical constants of [α]15D +15.5° (c=0.775) and [η]=20 (c=g./100 cc.), and was positive to Molisch, Bial, and naphthoresorcinol reactions, but negative to iodine, biuret and Ninhydrin reactions. On infrared spectrum, its potassium salt showed the presence of carboxylate ion.

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