A Bioluminescent Assay System for Whole-Cell Determination of Hormones

DOI Web Site Web Site PubMed 被引用文献3件 参考文献15件
  • Kim Sung Bae
    Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)
  • Suzuki Toshiyuki
    IBEC center, National Institute of Advanced Industrial Science and Technology (AIST)
  • Kimura Akira
    Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST)

この論文をさがす

抄録

A bioluminescent-assay system was fabricated for an efficient determination of bioactive small molecules in physiological samples. The following three components were newly created for this assay system: (i) a single-chain probe exerting a 7.2-times stronger optical intensity than conventional ones, (ii) a high throughput assay device uniquely designed for the assay system with ca. one-fourth smaller standard deviation (S.D.) to samples than without the device, (iii) a buffer cocktail optimized for the assay system. The advantages of the assay system were evaluated by determining (i) the stress hormone levels in human saliva and (ii) multicolor imaging of genomic and nongenomic effects of woman sex hormones. This study guides on how to fabricate an efficient assay system for bioactive small molecules with convenience and high precision.

収録刊行物

被引用文献 (3)*注記

もっと見る

参考文献 (15)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ