A New Approach to Characterization of Insulin Derived from Different Species Using 1H-NMR Coupled with Multivariate Analysis
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- Ohno Akiko
- Division of Organic Chemistry, National Institute of Health Sciences
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- Kawanishi Toru
- Division of Drugs, National Institute of Health Sciences
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- Okuda Haruhiro
- Division of Organic Chemistry, National Institute of Health Sciences
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- Fukuhara Kiyoshi
- Division of Organic Chemistry, National Institute of Health Sciences
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説明
Most of the active components of polypeptides have a complex molecular structure, large molecular size. Such components may also be structurally heterogeneous. Therefore, development of a method that can confirm the consistency of polypeptides amino-acid sequences for product characterization is desirable. In general, it is extremely difficult to distinguish differences of a few amino acid residues in the 1H-NMR spectrum of polypeptides with molecular weights greater than several thousand. However, we have been able to distinguish between three insulin species differing in one to three amino acid residues using a combination of multivariate statistics and 1H-NMR spectra. These results demonstrate that this methodology could be useful for characterization of polypeptides.
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 60 (3), 320-324, 2012
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679155070464
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- NII論文ID
- 130001852416
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- NII書誌ID
- AA00602100
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- COI
- 1:STN:280:DC%2BC383nvVWmtg%3D%3D
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 023439095
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- PubMed
- 22382411
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可