Chemical Ligation Reactions of Oligonucleotides for Biological and Medicinal Applications
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- Abe Hiroshi
- Department of Chemistry, Graduate School of Science, Nagoya University
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- Kimura Yasuaki
- Department of Chemistry, Graduate School of Science, Nagoya University
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<p>Chemical ligation of oligonucleotides (ONs) is the key reaction for various ON-based technologies. We have tried to solve the problems of RNA interference (RNAi) technology by applying ON chemical ligation to RNAi. We designed a new RNAi system, called intracellular buildup RNAi (IBR-RNAi), where the RNA fragments are built up into active small-interference RNA (siRNA) in cells through a chemical ligation reaction. Using the phosphorothioate and iodoacetyl groups as reactive functional groups for the ligation, we achieved RNAi effects without inducing immune responses. Additionally, we developed a new chemical ligation for IBR-RNAi, which affords a more native-like structure in the ligated product. The new ligation method should be useful not only for IBR-RNAi but also for the chemical synthesis of biofunctional ONs.</p>
収録刊行物
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- CHEMICAL & PHARMACEUTICAL BULLETIN
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CHEMICAL & PHARMACEUTICAL BULLETIN 66 (2), 117-122, 2018
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679155657472
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- NII論文ID
- 130006328058
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- NII書誌ID
- AA00602100
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- ISSN
- 13475223
- 00092363
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- NDL書誌ID
- 028787362
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- PubMed
- 29386461
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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- KAKEN
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- 抄録ライセンスフラグ
- 使用不可