Inhibition of alanine aminotransferase activity by IgG.
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- Ando Toshiko
- Department of Clinical Laboratories, Yokohama City University Hospital
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- Watanabe Shinichiro
- Department of Clinical Laboratories, Yokohama City University Hospital
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- Okawa Kiyo
- Department of Clinical Laboratories, Yokohama City University Hospital
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- Ogawa Noboru
- Department of Clinical Laboratories, Yokohama City University Hospital
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- Ehara Shigeru
- Department of Clinical Laboratories, Yokohama City University Hospital
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- Nagasawa Yoshimi
- 健康保険組合連合会大阪中央病院中央検査部
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- Ishihara Jyun
- 静岡県立こども病院臨床病理科
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- Kameko Fumiko
- 信州大学医学部保健学科検査技術科学専攻
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- Fujita Kiyotaka
- 信州大学医学部保健学科検査技術科学専攻
Bibliographic Information
- Other Title
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- ALT活性阻害を示すIgGの免疫化学的特性
- ALT カッセイ ソガイ オ シメス IgG ノ メンエキ カガクテキ トクセイ
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Description
We observed a 74-year old woman with chronic hepatitis C, whose alanine aminotransferase (L-Alanine: 2-oxoglutarate aminotransferase: EC.2.6. 1.2; ALT) activity in serum was below 2 IU/L (reference value: 9-25 IU/L). ALT activity in normal human serum was inhibited when the patient's serum was added to normal serum. By immunoprecipitation assay, it was found that the patient's IgG-κ acted as an inhibitor of ALT. The ALT inhibition of the patient's IgG was not blocked by adding alanine as a substrate, and also by adding pyridoxal phosphate as a coenzyme. The purified patient's IgG was found to be composed of two γ chains of about 50kDa and two κ chains of 28kDa by SDS-PAGE. The inhibitory ability of IgG was exclusively located in the Fab region of the molecule.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 50 (4), 225-229, 2006
Japanese Electrophoresis Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282679177498880
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- NII Article ID
- 10018441597
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- NII Book ID
- AN00129729
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- ISSN
- 13499785
- 00319082
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- NDL BIB ID
- 8615624
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed