Immunochemical confirmation of migration of albumen gland proteins to the egg of the land snail, Euhadra: Analysis of the proteins by two-dimensional gel electrophoresis and Western blotting method.

DOI Open Access
  • Asaga Hiroaki
    Department of Biology, Tokyo Gakugei University Department of Biology, Faculty of Science, Tokyo Metropolitan University
  • Kozawa Satoko
    Department of Biology, Tokyo Gakugei University
  • Ebara Atsushi
    Department of Biology, Tokyo Gakugei University
  • Inoue Tsutomu
    Department of Biology, Tokyo Gakugei University

Bibliographic Information

Other Title
  • <i>Euhadra</i> 属における卵白腺から卵への蛋白質の移行の免疫生化学的確証
  • Analysis of the proteins by two-dimensional gel electrophoresis and Western blotting method

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Description

To confirm the migration of proteins from the albumen gland to the egg of the land snail, Euhadra, the soluble proteins extracted from albumen glands and eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed immunochemically.<br>The major polypeptide groups (named No. 8 and 9 polypeptide groups on the polypeptide map) of soluble proteins extracted from the albumen gland of E. peliomphala were purified and used to immunize rabbits. The specific antibodies against the albumen gland proteins (anti-AG) were purified from the antiserum. The soluble proteins extracted from the albumen glands and the eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed by O'Farrell's two-dimensional gel electrophoresis and Western blotting method with anti-AG. The anti-AG reacted not only with the two polypeptide groups, which were the same as the immunogens used, but also with another polypeptide group (named No. 14 polypeptide group on the polypeptide map) of all the samples tested. These results support strongly the migration of these polypeptide groups from the albumen gland to egg in Euhadra.

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Details 詳細情報について

  • CRID
    1390282679177554304
  • NII Article ID
    130003606830
  • DOI
    10.2198/sbk.32.135
  • ISSN
    13499785
    00319082
  • Text Lang
    en
  • Data Source
    • JaLC
    • Crossref
    • CiNii Articles
    • OpenAIRE
  • Abstract License Flag
    Disallowed

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