Immunochemical confirmation of migration of albumen gland proteins to the egg of the land snail, Euhadra: Analysis of the proteins by two-dimensional gel electrophoresis and Western blotting method.

Asaga Hiroaki, Kozawa Satoko, Ebara Atsushi, Inoue Tsutomu

doi:10.2198/sbk.32.135

Bibliographic Information

Other Title

<i>Euhadra</i> 属における卵白腺から卵への蛋白質の移行の免疫生化学的確証
Analysis of the proteins by two-dimensional gel electrophoresis and Western blotting method

Description

To confirm the migration of proteins from the albumen gland to the egg of the land snail, Euhadra, the soluble proteins extracted from albumen glands and eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed immunochemically.<br>The major polypeptide groups (named No. 8 and 9 polypeptide groups on the polypeptide map) of soluble proteins extracted from the albumen gland of E. peliomphala were purified and used to immunize rabbits. The specific antibodies against the albumen gland proteins (anti-AG) were purified from the antiserum. The soluble proteins extracted from the albumen glands and the eggs of E. peliomphala, E. subnimbosa and E. quaesita were analyzed by O'Farrell's two-dimensional gel electrophoresis and Western blotting method with anti-AG. The anti-AG reacted not only with the two polypeptide groups, which were the same as the immunogens used, but also with another polypeptide group (named No. 14 polypeptide group on the polypeptide map) of all the samples tested. These results support strongly the migration of these polypeptide groups from the albumen gland to egg in Euhadra.

Journal

SEIBUTSU BUTSURI KAGAKU

SEIBUTSU BUTSURI KAGAKU 32 (3), 135-140, 1988

Japanese Electrophoresis Society

Keywords

Details 詳細情報について

CRID: 1390282679177554304

NII Article ID: 130003606830

DOI: 10.2198/sbk.32.135

ISSN: 13499785; 00319082

Text Lang: en

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