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- 長束 俊治
- 京都工芸繊維大学繊維学部応用生物学科
書誌事項
- タイトル別名
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- Analysis of glycan expression by fluorescence labeling method.
- ケイコウ ヒョウシキ ニ ヨル トウサ ノ ハツゲン パターン カイセキホウ
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The pyridylamination method was originally described in 1978 as a means of analyzing glycan structures with high-sensitivity. Subsequently, the method has been applied to structure analyses of glycans including glycosidase digestion, 2D-mapping by various kinds of HPLC's, partial acetolysis, Smith degradation, methylation analysis, nuclear magnetic resonance, mass spectrometry, and affinity assay for lectins. Glycans on glycoconjugates are liberated by hydrazinolysis followed by N-acetylation. Reducing ends of the released glycans are tagged with 2-aminopyridine by reductive amination. Pyridylamino (PA-) derivatives of glycans with fluorescence and a positive charge have the following advantages: 1. high sensitivity in detection; 2. excellent separation in reversed phase HPLC; 3. high chemical stability under the conditions for structure elucidation; 4. applicable to many authentic methods for glycan analysis.
収録刊行物
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- 生物物理化学
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生物物理化学 46 (2), 35-38, 2002
日本電気泳動学会
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詳細情報 詳細情報について
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- CRID
- 1390282679178117632
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- NII論文ID
- 10012731392
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- NII書誌ID
- AN00129729
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- ISSN
- 13499785
- 00319082
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- NDL書誌ID
- 6209723
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
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- 抄録ライセンスフラグ
- 使用不可