リポソームをリガンドとして用いた血漿蛋白質のプロテオミクス解析

DOI Web Site 被引用文献1件 参考文献14件

書誌事項

タイトル別名
  • Plasma proteomic analysis using liposome as a biological ligand.
  • リボソーム オ リガンド ト シテ モチイタ ケッショウ タンパクシツ ノ プロテオミクス カイセキ

この論文をさがす

抄録

Human plasma contains a numerous number of proteins that provide variable information reflecting to physiological and pathological conditions of human body. Some of such proteins have been expected for pathobiological markers for diagnosis and drug development for human diseases. To capture plasma proteins in a manner of high-through put, several ligands have been employed, though these systems are still required many improvements. In this report, we present a novel system using liposome as a ligand to capture a wide variety of plasma proteins. One hundred micro litter of plasma was incubated with liposome of multi-lamellar vesicle in the presence of Ca2+. Proteins bound to liposome were then eluted in the presence of EGTA. A two-dimensional gel analysis revealed more than 150 proteins in one electrophoresis run in a range of pH 4-7 with a molecular weight between 100kda to 30kda. The pattern of silver-stained proteins showed reproducible with the same donor. Apparent differences were detected between the pattern under different pathologic conditions including healthy volunteer and that with patients with chronic inflammation. Taken together, the method using liposome might provide a useful biological ligand for plasma protein analysis and a novel system for proteomic approach combined with other chromatographic module.

収録刊行物

被引用文献 (1)*注記

もっと見る

参考文献 (14)*注記

もっと見る

関連プロジェクト

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ