An improved silver staining method for high resolution agarose gel electrophoresis

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  • 高分解能アガロースゲル電気泳動法における銀染色改良法の基礎的研究

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Recently, silver staining has been used for the detection of a trace amount of protein using agarose gel electrophoresis. However, some problems, such as easy contamination of the background by nonprotein substances and necessity of complicated steps and skillful technique for staining have made it less satisfactory. A more simplified method is necessary for routine work. Therefore, we modified successfully the silver staining method published by Kerényi et al.<br>The present improved silver staining method gave the same sensitivity as Kerényi's method, and yet the procedure was simplified and the staining reproducibility was satisfactory. It is also useful for the detection of oligoclonal bands in cerebrospinal fluid from the patients with demyelinating diseases and various types of viral infections, when it is combined with high resolution agarose gel.

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