書誌事項
- タイトル別名
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- Agarose-2D-DIGE: Fluorescent 2-D differential gel electrophoresis (2-D DIGE) with agarose gels in the first-dimensional isoelectric focusing (IEF)
- 1ジゲンメ ニ アガロース ゲル トウデンテン デンキ エイドウ オ モチイタ ケイコウ ディファレンス ゲル 2ジゲン デンキ エイドウ
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説明
A two-dimensional gel electrophoresis (2-DE) method that uses an agarose isoelectric focusing (IEF) gel in the first dimension (agarose 2-DE) offers advantages over the more widely used immobilized pH gradient 2-DE for separating high-molecular-mass proteins (100-500kDa) and for having a higher loading capacity (1.5mg in total). We have applied the Fluorescent 2-D differential gel electrophoresis (2-D DIGE) to our agarose 2-DE system. This allowed us to see clear differences in the 2-DE patterns from liver extracts of a diabetic model Otsuka Long-Evans Tokushima Fatty (OLETF) and its control Long-Evans Tokushima Otsuka (LETO) rats including changes in the amounts of several proteins larger than 100kDa. The combined method would increase its power to detect changes in disease proteomics.
収録刊行物
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- 生物物理化学
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生物物理化学 50 (3Special), 173-178, 2006
日本電気泳動学会
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詳細情報 詳細情報について
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- CRID
- 1390282679178540160
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- NII論文ID
- 10018760579
- 130003607378
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- NII書誌ID
- AN00129729
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- ISSN
- 13499785
- 00319082
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- NDL書誌ID
- 8528104
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