Purification and properties of .ALPHA.1- and .ALPHA.2-macroglobulins in the dog.
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- Sakaguchi Kazuko
- College of Environmental Health, Azabu University
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- Akahori Fumiaki
- School of Veterinary Medicine, Azabu University
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- Hamada Kimio
- School of Veterinary Medicine, Azabu University
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- Kishi Miki
- College of Environmental Health, Azabu University
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- Masaoka Toshio
- School of Veterinary Medicine, Azabu University
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- Shirai Mitsuyuki
- School of Veterinary Medicine, Azabu University
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- Kohzaki Ryuko
- College of Environmental Health, Azabu University
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- Sawada Atsushi
- College of Environmental Health, Azabu University
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- Kohzaki Ken-ichi
- School of Veterinary Medicine, Azabu University
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- Kobayashi Sadao
- College of Environmental Health, Azabu University
Abstract
1) The ultrafiltration of serum collected from dogs by a filtration film with a pore size of 300, 000, Blue Sepharose CL-6B affinity chromatography and Ultrogel AcA 22gel filtration chromatography eluted a third protein fraction in addition to α1-macroglobulin (α1-M) and α2-macrogrobulin (α2-M). The subsequent use of preparative agarose gel isoelectric focusing with a cover gel facilitated the extraction of pure α1-M and α2-M protein fractions.2) Analytical agarose gel isoelectric focusing and Ferguson plot by agarose gel electrophoresis revealed the following: (1) dog α1-M had an isoelectric point (pI) of 5.4 and a molecular weight of 724, 000; and (2) dog α2-M had a pI of 5.7 and a molecular weight of 758, 000.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 36 (2), 139-143, 1992
Japanese Electrophoresis Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282679178644736
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- NII Article ID
- 130003606935
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- ISSN
- 13499785
- 00319082
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed