Mass spectrometric analysis of phospholipids with phosphate monoester residue using Phos-tag
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- Tanaka Tamotsu
- Institute of Health Biosciences, University of Tokushima Graduate School
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- Morishige Jun-ichi
- Research Center for Green Science, Fukuyama University
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- Urikura Mai
- Department of Applied Biological Science, Fukuyama University
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- Tokumura Akira
- Institute of Health Biosciences, University of Tokushima Graduate School
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- Satouchi Kiyoshi
- Department of Applied Biological Science, Fukuyama University
Bibliographic Information
- Other Title
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- Ｐｈｏｓ－ｔａｇを用いた活性リン脂質の質量分析
- Phos-tag オ モチイタ カッセイ リン シシツ ノ シツリョウ ブンセキ
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Abstract
Lysophosphatidic acid (LPA) and sphingosine 1-phosphate (S1P) are growth factor-like bioactive lipids having a phosphate monoester residue. Phos-tag can bind to them and be used both for purification and quantification. In a two-phase solvent system consisting of chloroform/methanol/water, addition of Phos-tag move LPA and S1P from a hydrophilic phase to a hydrophobic phase in the form of their Phos-tag complexes. Using this property, we developed a method for purification of LPA and S1P in biological materials by the phase separation technique. Advantages of use of Phos-tag for detection of LPA and S1P in MALDI-TOF MS are an increase in ionization efficiency and detection as a single-ion form. Homologues of LPA and S1P in natural samples can be quantified by MALDI-TOF MS by using internal standards.<br>
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 56 (Suppl_1), S37-S40, 2012
Japanese Electrophoresis Society
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Details 詳細情報について
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- CRID
- 1390282679178944384
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- NII Article ID
- 130004809454
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- NII Book ID
- AN00129729
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- ISSN
- 13499785
- 00319082
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- NDL BIB ID
- 023553759
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- Text Lang
- ja
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed