Color developmental conditions for LDH isoenzyme assay by agarose gel electrophoresis.
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- Kuroda Itsuo
- Federation of National Public Service Porsonnel Mutual Aid Association, Tachikawa Hospital
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- Nakazawa Chinami
- Federation of National Public Service Porsonnel Mutual Aid Association, Tachikawa Hospital
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- Mori Kohsuke
- 国家公務員等共済組合連合会立川病院・内科
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- Yoshida Mitsutaka
- 東邦大学・理学部生理化学
Bibliographic Information
- Other Title
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- アガロースゲル電気泳動におけるLDHアイソザイムの発色条件の改良
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Abstract
Color developmental condition in LDH isoenzyme assay using agarose gel film as medium was investigated fundamentally. Optimum conditions for this assay were pH8.6, DL-litium lactate concentration 120∼150mM/l, NAD concentration 4∼5mM/l and tricine concentration 0.1M/l. These conditions were found to be identical for those of Pol-E film method. However, optimum concentrations of diaphorase enzyme used as an intermediate electron carrier was 7, 500U/l and that of NTB was 0.5mM/l. Furthermore, the color developmental reaction in agarose gel film was a linear at least in 60 minutes. After these improvements, an assay condition with better color developmental sensibility and quantitability was obtained in comparison with Pol-E film method.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 29 (6), 403-406, 1985
Japanese Electrophoresis Society
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Keywords
Details 詳細情報について
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- CRID
- 1390282679179038336
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- NII Article ID
- 130003606720
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- ISSN
- 13499785
- 00319082
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed