Electroendosmotic liquid flows in various wide and narrow pH range isoelectric focusing gel prepared with IsoGel and Agarose IEF.

DOI Open Access
  • Kobayashi Sadao
    Department of Bioorganic Chemistry, College of Environmental Health, Azabu University
  • Yoshihara Eiji
    Department of Bioorganic Chemistry, College of Environmental Health, Azabu University
  • Suzuki Jun
    Department of Bioorganic Chemistry, College of Environmental Health, Azabu University

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Other Title
  • 広域および狭域両性担体を用いた薄層アガロース等電点電気泳動における液流

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The electroendosmotic behavior of IsoGel and Agarose IEF was determined in a series of various wide and narrow pH range electrofocusing experiments in which glucose served as marker substance.<br>In IsoGel its liquid flow caused towards the anode in all wide and narrow pH range electrofocusing. The strength of segmetal liquid flow was proportional to voltage distribution across the gel. The pH gradients obtained in IsoGel when using same wide pH range ampholyte has markedly low pH values than that of Agerose IEF at acidic region.<br>In Agarose IEF its liquid fow behavior differed in wide and narrow pH range electrofocusing. In the case of wide pH range electrofocusing its liquid flow reversed towards the anode at pH 5.5∼6 region in the foucsed gels. The other hand, in narrow pH range electrofocusing the direction of liquid flow was differed with pH range of ampholyte. In higher pH range than Amopholine 5-7 its liquid flow coused towards the cathode and in lower pH range than Ampholine 4-6 reversed towards the anode. The direction and the streght of liquid flow in narrow pH range Agarose IEF depends on the pH distribution in the focused gel. The pH curves obtained in Agarose IEF when using same narrow pH range Ampholyte were slightly higher than that of IsoGel.

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