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Electrophoretic analysis of serum ultrafiltrate obtained by ECUM from hemodialysis patient.
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- Honma Nobuyuki
- Department of Chemistry, Faculty of Science, Niigata University
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- Sugano Hiroshi
- Department of Chemistry, Faculty of Science, Niigata University
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- Gejyo Fumitake
- 新潟大学医学部第2内科
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- Arakawa Masaaki
- 新潟大学医学部第2内科
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- Itoo Giichi
- 新潟大学保健管理センター
Bibliographic Information
- Other Title
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- 血液透析患者から得たECUMろ過液のSDS‐PAGE分析
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Description
Serum ultrafiltrate was obtained from hemodialysis patients by using an extra corporeal ultrafiltration method equipped with a hollow fiber dialyser, and electrophoresed on the SDS-polyacrylamide gel having a high resolving power in the low molecular weight region. Among the major bands less than 40KD observed with plasma of hemodialysis patient, the bands of 29K, 28K, 25K, 22K, 16.7K and 8.9K molecular weights were also found in serum ultrafiltrate of the same patient. By an immunological method, the following bands were identified: α1-microglobulin (28K), retinol-binding protein (22K), prealbumin (16.7K) and β2-microglobulin (8.9K). As compared with serum ultrafiltrate obtained from healthy person, 28K, 25K (in some cases), 22K and 8.9K bands showed obvious increase in cases of hemodialysis patients except for one patient who showed an urine volume of 200ml/day and an electrophoretic pattern closed to that of healthy person.
Journal
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- SEIBUTSU BUTSURI KAGAKU
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SEIBUTSU BUTSURI KAGAKU 30 (4), 283-288, 1986
Japanese Electrophoresis Society
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Details 詳細情報について
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- CRID
- 1390282679180032512
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- NII Article ID
- 130003606760
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- ISSN
- 13499785
- 00319082
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
- OpenAIRE
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- Abstract License Flag
- Disallowed