Differentiation of neural crest cells from spheres of mouse ES cells cultured in a serum-free culture

DOI Web Site 13 References
  • Minamino Yuki
    Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University
  • Ohnishi Yuichi
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • Watanabe Masahiro
    Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University
  • Ugaki Yoshihide
    Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University
  • Hamada Machi
    Graduate School of Dentistry (Second Department of Oral and Maxillofacial Surgery), Osaka Dental University
  • Inoue Hiroshi
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • Dateoka Suguru
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • Yunoki Hirokazu
    Department of Dentistry and Maxillofacial Surgery, Ishinkai Yao General Hospital
  • Nakajima Masahiro
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
  • Kakudo Kenji
    Second Department of Oral and Maxillofacial Surgery, Osaka Dental University

Bibliographic Information

Other Title
  • 無血清培養系を用いたマウスES細胞からのneural crest細胞への分化誘導
  • ムケッセイ バイヨウケイ オ モチイタ マウス ES サイボウ カラ ノ neural crest サイボウ エ ノ ブンカ ユウドウ

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Abstract

Recently, the use of multipotent stem cells has been studied as for regeneration medicine of missing craniofacial bone. Most craniofacial structures are derived from cranial neural crest cells. We investigated the use of craniofacial bone induction from mouse embryonic stem (ES) cells differentiated into neural crest cells via neuroectoderm. First, we cultured mouse ES cells in serum-free neuroinduction medium for sphere formation and confirmed they were precursors of neuronal and neural crest cells by immunostaining using specific antibodies for Nestin and AP2α, respectively. The sphere contained Nestin and AP2α positive cells, indicating that a portion of the ES cells differentiated into neural crest cells through neural precoursor cells during sphere formation. These findings indicated the possibility that neural crest cells can be generated from mouse ES cells through spheres cultured in serum-free medium.

Journal

  • Shikaigaku

    Shikaigaku 74 (2), 35-39, 2011

    Osaka Odontological Society

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