The parasitic cycle of Coccidioides immitis in murine coccidioidomycosis.

DOI Open Access
  • Miyaji Makoto
    Department of Pathogenic Fungi, Research Institute for Chemobiodynamics, Chiba University
  • Nishimura Kazuko
    Department of Pathogenic Fungi, Research Institute for Chemobiodynamics, Chiba University
  • Terao Kiyoshi
    Department of Toxicology and Experimental Pathology, Research Institute for Chemobiodynamics, Chiba University

Bibliographic Information

Other Title
  • Ｃｏｃｃｉｄｉｏｉｄｅｓ　ｉｍｍｉｔｉｓのマウス組織内におけるｐａｒａｓｉｔｉｃ　ｃｙｃｌｅ

Search this article

Description

The parasitic cycle of Coccidioides immitis in murine coccidioidomycosis was investigated using a scanning electron microscope. An arthroconidium suspension containing 3.3×105 arthroconidia in 0.1ml phosphate buffered saline was prepared from a culture of C. immitis IFM 4935 incubated at 37°C for 14 days. As experimental animals, 7 congenitally athymic nude mice and 7 of their heterozygous littermates were used. Each mouse was inoculated intravenously with 0.1ml of the arthroconidium suspension and dessected 3, 5, and 6 days after inoculation.<br>The liver was severely affected by the fungus, followed by the spleen and lungs in that order. The inoculated arthroconidia swelled and became spherical. Many young spherules with or without cellular responses were observed in the liver of both groups of mice 3 days after inoculation. On that day a few spherules reached the midpoint stae of their parasitic cycle. All the developmental stages, from spherule initials to released endospores, were found in each specimen prepared from the tissue of both groups of mice on the 5th and 6th day after inoculation. These developmental stages were clearly demonstrated by scanning electron microscopy.

Journal

Details 詳細情報について

Report a problem

Back to top