5′-イノシン酸ナトリウムの簡易比色法 5′-リボヌクレオチドの安定性に関する研究 (第5報)

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タイトル別名
  • A Simplified Colorimetry Procedure for Sodium 5′-Inosinate Determination
  • 5 イノシンサン ナトリウム ノ カンイ ヒショクホウ

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説明

A simplified colorimetry procedure was developed for the determination of sodium 5′-inosinate (5′-IMP) in sodium 5′-ribonucleotide (5′-Rb) and in flavor-enhancing preparations consisting of monosodium L-glutamate (92-98.5%) and 5′-Rb (8-1.5%).<br>In the present method, 5′-IMP was decomposed to diazotizable amine (DA) by treatment with zinc powder in dilute hydrochloric acid. The DA formed a reddish-purple-colored derivative with the Bratton-Marshall reagent, with an absorption maximum at 540nm. A linear relationship was observed between absorbance at 540nm and concentration of 5′-IMP in the range of 5-50μg/ml.<br>Interfering substances in the present method were similar to those in the official method for the determination of 5′-IMP in 5′-Rb or in flavor-enhancing preparations.<br>The time required for assay by the present method was reduced to about one-third of that required by the official method.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 21 (5), 381-385_1, 1980

    公益社団法人 日本食品衛生学会

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