Development of Event-Specific Quantitation Method for GA21 Maize, Which Is a GM Event without CaMV35S Promoter
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- OGUCHI Taichi
- National Agriculture and Food Research Organization, National Food Research Institute
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- ONISHI Mari
- Fasmac Co., Ltd.
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- CHIKAGAWA Yukie
- Fasmac Co., Ltd.
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- MINEGISHI Yasutaka
- Nippon Gene Co., Ltd.
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- KODAMA Takashi
- Food and Agricultural Materials Inspection Center
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- AKIYAMA Hiroshi
- National Institute of Health Sciences
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- OHNO Yasuo
- National Institute of Health Sciences
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- FUTO Satoshi
- Fasmac Co., Ltd.
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- HINO Akihiro
- National Agriculture and Food Research Organization, National Food Research Institute
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- FURUI Satoshi
- National Agriculture and Food Research Organization, National Food Research Institute
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- KITTA Kazumi
- National Agriculture and Food Research Organization, National Food Research Institute
Bibliographic Information
- Other Title
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- CaMV35Sプロモータ配列を持たない遺伝子組換えトウモロコシGA21系統の系統特異的定量分析法の開発
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Description
A real-time PCR detection method was developed for event-specific quantitation of Roundup Ready® maize, GA21. The developed PCR method was designed to amplify an artificial junction site between the native maize genome DNA and the recombinant DNA of GA21 maize, which provides only one target sequence per haploid of GA21 genome. Thus, the amplification efficiency of the event-specific target for GA21 became closely similar to the amplification of SSIIb, and the conversion factor (Cf) for the quantitation method was similar to the theoretical value. The developed method demonstrated better performance than the existing construct-specific method that has been used as a Japanese official method. The developed method can easily be combined with the real-time PCR targeting of the CaMV35S promoter, and the multiplexed method should be an effective screening method for GM maize.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 49 (1), 16-22, 2008-02-29
Japanese Society for Food Hygiene and Safety
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Keywords
Details 詳細情報について
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- CRID
- 1390282679200637440
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- NII Article ID
- 130000054303
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 9414945
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed