On the Reduction of Janus Green B by Plant Embryo Slices and the Mechanism of the Specific Stainiug of Mitochondria

SATO Sitiro

doi:10.15281/jplantres1887.69.87

Bibliographic Information

Other Title

組織切片によるャヌスグリーンB還元とミトコンドリャ染色の機構について

Abstract

Janus green B stains the periderm and the pith of hypocotyl and radicle of ungerminated seeds of Phaseolus vulgaris. The procambium, which contains no mitochondria, is not stained from beginning to end of the procedure unless the cells are living Under a coverglass, the stain reduced and decolorized. This reduction is inhibited by heating (52°C for 30min.), low pH, acetate buffer (M/10), malonate monoiodoacetate (M/1000), arsenite (M/300), and Pb (NO³)² (M/10), and accelerated (M/100), by succinate (6M/10). These inhibitions and acceleration show that succinic dehydrogenase plays a role in the process of the reduction of Janus green B, presumably in directly under the present experimental conditions. Cyanide (M/1000) also inhibits the reduction. Without coverglass, i. e. in complete aerobic condition, the reduction dose not occur whether cyanide is present or not.<br>The hypothesis of L Aazarow and Cooperstein on the mechanism of the specific staninig of mitochondria was criticized on the ground of the above stated experimental data and some theoretical considerations.

Journal

Shokubutsugaku Zasshi

Shokubutsugaku Zasshi 69 (812), 87-90, 1956

The Botanical Society of Japan

Details 詳細情報について

CRID: 1390282679201126400

NII Article ID: 130004212294

DOI: 10.15281/jplantres1887.69.87

ISSN: 21853835; 0006808X

Data Source

JaLC
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CiNii Articles

Abstract License Flag: Disallowed

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