Bactericidal Effect of Peracetic Acid on <i>Bacillus subtilis</i> Spore

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  • 過酢酸の <i>B. subtilis</i> 胞子に対する殺菌効果
  • カサクサン ノ B.subtilisホウシ ニ タイスル サッキン コウカ

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The bactericidal effect of peracetic acid on Bacillus subtilis (ATCC 6633) spores was investigated. The survival curves of spores were analyzed, and the D value at 25°C was found to be 2.4 (minutes) for 2.0×10-3M peracetic acid. The concentration index was found to be 1.70 from D values in the concentration range of 0.5-5.0×10-3M peracetic acid at 25°C, and the temperature quotient (Q10) was found to be 2.66 from D values at 15-35°C, for 2.0×10-3M peracetic acid.<br>Almost the same level of bactericidal effect was obtained in the range of pH 3.6-7.2, but a decrease of bactericidal effect was observed at pH 7.8. The bactericidal action was apparently not affected by hydrogen peroxide at low concentrations. D values for P3- oxonia aktiv (OA) solution agreed closely with those for corresponding concentrations of peracetic acid.<br>After CIP circulation several times, it was observed that the concentration of peracetic acid was decreased much more than that of hydrogen peroxide in OA solution, because of the lower stability of peracetic acid. It was necessary to maintain the initial concentration of peracetic acid for repeat use of OA solution in order to prevent a decrease of bactericidal effect.

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