消化酵素に対する食用色素の作用 (第2報)

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書誌事項

タイトル別名
  • Action of Food Dyes on Digestive Enzymes (II)
  • ショウカ コウソ ニ タイスル ショクヨウ シキソ ノ サヨウ 2 キモトリプ
  • Interaction of Xanthene Dyes with Chymotrypsinogen
  • キモトリプシノーゲンとキサンテン系色素の相互作用

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抄録

Inactivation of chymotrypsinogen in the presence of xanthene dyes such as rose bengale, phloxine, erythrosine and eosine was observed through the irradiation of light, but not in the dark. The extent of inhibition was in the following order; rose bengale>phloxine>erythrosine>eosine.<br>Interaction of xanthene dyes with chymotrypsinogen were also examined by the spectrophotometric methods. In the presence of chymotrypsinogen, the absorption maxima of the dyes did not shift at pH 6.97, 7.99 and 10.0, but shifted to longer wavelength at pH 4.73 and 5.78.<br>The binding numbers and the binding free energies were calculated from the difference spectra for the mixtures of the dyes and chymotrypsinogen which were incubated at 37°C for 15min. In comparison the both indexes of rose bengale to chymotrypsinogen in the dark with those in the light, they were not so increased in proportion to the inactivation of chymotrypsinogen. The order of binding capacities and affinities was rose bengale>phloxine>erythrosine>eosine at pH 4.77, regardless of the conditions with and without the irradiation.<br>It was assumed that chymotrypsinogen would be inactivated in the presence of xanthene dyes by the photo-effect, not by the binding capacities and/or the affinities.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 18 (6), 493-499_1, 1977

    公益社団法人 日本食品衛生学会

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