Hemolysis by Detergents and their Effects on Glycolysis in Human Erythrocytes

DOI Web Site

Bibliographic Information

Other Title
  • 合成洗剤のヒト赤血球の溶血性および解糖系に及ぼす影響
  • ゴウセイ センザイ ノ ヒト セッケッキュウ ノ ヨウケツセイ オヨビ カイト

Search this article

Description

As a part of the study on safety evaluation of detergents, the hemolytic activity and the effect on glycolysis in human erythrocytes were investigated.<br>Fifty per cent hemolysis by various detergents occurred in the range from 7 to 73ppm in low blood concentration, but the hemolytic activity in high blood concentration (50v/v%) was decreased to about one-hundredth. The order of hemolytic activity was almost parallel to the lowering activity of surface-tension. In general, the hemolysis by anionic detergents was more potent than that of nonionic ones.<br>The glycolysis in erythrocytes was not influenced by treatment with various detergents at the concentration of one-fourth of 50% hemolytic value. However, the glycolysis in burst erythrocytes was inhibited from 50 to 70% by the anionic and stimulated from 30 to 100% by the nonionic, at 10-3M concentration of detergents. From the studies with some glycolytic enzymes, it was suggested that the inhibition of glycolysis by anionic detergents was due to the formation of the enzyme–detergent complex, and the stimulation by nonionic detergents was attributed to the stimulation of aldolase activity.

Journal

Keywords

Details 詳細情報について

Report a problem

Back to top