Simultaneous Determination of Theobromine, Theophylline and Caffeine in Foods by High Performance Liquid Chromatography

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  • 高速液体クロマトグラフィーによる食品中のテオブロミン, テオフィリン及びカフェインの同時分析法
  • コウソク エキタイ クロマトグラフィー ニ ヨル ショクヒンチュウ ノ テオブ

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A method for the simultaneous determination of theobromine, theophylline and caffeine in several kinds of foods is described.<br>The chromatographic separation of the three compounds was achieved on FLC-ODS employing water-acetonitrile (150:10) as the mobile phase. The methylxanthines in tea and coffee were extracted with water and in turn the aqueous layer was extracted with chloroform. Clean-up of these compounds in cocoa and chocolate was efficiently attained by extracting them with water in the presence of carbon tetrachloride.<br>The recovery rates of standard samples added to foods were 88-96%.

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