変敗油中のマロンアルデヒドのけい光分析

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タイトル別名
  • Fluorometric Assay of Malonaldehyde in Autoxidized Oil
  • ヘンパイユチュウ ノ マロンアルデヒド ノ ケイコウ ブンセキ エイブン

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抄録

Fluorometric assay of malonaldehyde in oxidized oil was investigated. The mixture of 20g of whale oil or rape seed oil and 20ml of n-hexane was prepared. To the mixture was added to 5ml of pH 7.8 phosphate buffered solution (ionic strength, 0.05) and was shaken for 10min at room temperature. The shaken mixture was centrifuged at 4, 000rpm for 20min and was separated the buffered solution extracts from the mixture. A part of the extract was reacted with DL-valine in 50% acetic acid (A), and the other part was added on a polyamide dry column and eluted with water. The eluate was reacted with DL-valine in 50% acetic acid (B). Fluorescence of (A) and (B) were measured at 460nm when samples were excited at 390nm. Malonaldehyde in oil was determined from (fluorescence (A)-fluorescence (B)). Malonaldehyde in rape seed oils was more than 1.9μg/kg.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 18 (5), 431-438_1, 1977

    公益社団法人 日本食品衛生学会

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