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- TAKEDA Yuiko
- National Institute of Hygienic Sciences
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- ISOHATA Etsuko
- National Institute of Hygienic Sciences
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- AMANO Ryuji
- National Institute of Hygienic Sciences
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- UCHIYAMA Mitsuru
- National Institute of Hygienic Sciences
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- NAOI Yasuta
- Metropolitan Research Laboratory of Public Health
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- NAKAO Mitsuo
- The Japan Food and Drug Safty Research Center
Bibliographic Information
- Other Title
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- マイコトキシンの化学分析に関する研究 (V)
- マイコトキシン ノ カガク ブンセキ ニカンスルケンキュウ 5 コクルイチュウ
- Systematic Analysis of Mycotoxins in Grains
- 穀類中のマイコトキシンの一斉分析について
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Description
Systematic analysis for the simultaneous determination of fluorescent mycotoxins, aflatoxin B1, B2, G1, G2, sterigmatocystin, ochratoxin-A and citrinin from polluted grains was studied.<br>The mycotoxins were extracted with the solvent consisted of 2ml of 20% sulfuric acid, 20ml of 4% potassium chloride solution and 178ml of acetonitrile. They were transfered to chloroform and freed from solvent. By means of silica gel column chromatography the former five toxins were cleaned-up and eluted by chloroform-methanol mixture (97:3) and the remaining ochratoxin-A and citrinin were recovered in the fraction eluted by benzene-acetone-acetic acid (75:20:5) after the intermediate washing of the column with chooroform-methanol (93:7).<br>Each fraction was concentrated and applied to thin layer chromatography for final determination, in which the mutual separation of ochratoxin-A and citrinin was performed by employing DC-Fertigplatten Kieselgel for solid layer and chloroform-methanol-water-90% formic acid (90:10:1:1) as developing solvent.<br>The fluorescent intensity of thin layer spots of sterigmatocystin, ochratoxin-A and citrinin were amplified by spraying 20% alminium trichloride solution, exposing in ammonia vaper, and spraying 20% alminium trichloride after exposure in ammonia vaper, respectively.
Journal
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- Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi)
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Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 17 (2), 193-199_1, 1976
Japanese Society for Food Hygiene and Safety
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Details 詳細情報について
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- CRID
- 1390282679201957376
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- NII Article ID
- 130003692011
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- NII Book ID
- AN00117741
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- ISSN
- 18821006
- 00156426
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- NDL BIB ID
- 1706832
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- Data Source
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- JaLC
- NDL Search
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed