Determination of Sodium Azide in Wine by High Performance Liquid Chromatography

DOI Web Site 1 Citations

Bibliographic Information

Other Title
  • 高速液体クロマトグラフィーによるワイン中のアジ化ナトリウムの分析法
  • コウソク エキタイ クロマトグラフィー ニ ヨル ワインチュウ ノ アジカ ナ

Search this article

Description

A sensitive and rapid method for the determination of sodium azide in wine by high performance liquid chromatography (HPLC) was developed.<br>Sodium azide in wine was converted into 3, 5-dinitrobenzoyl azide (DNBA) by reaction with 3, 5-dinitrobenzoyl chloride (DNBC). The reaction mixture was passed through a Seppak Florisil cartridge. The cartrigde was washed with water, then DNBA was eluted with a mixture of acetonitrile-0.05M citrate buffer solution, pH 3.6 (1:1). The eluate was injected onto a Finepak SIL C18 column and eluted with a mixture of acetonitrile-water (1:1) as the mobile phase, and detected with a UV detector set at 254nm.<br>The recoveries of sodium azide added to white wine, red wine and sparkling wine were more than 94%. The detection limit of sodium azide in wine was 0.05μg/ml. The total analysis time was 40 minutes per sample.<br>By using this method, 52 commercial samples were analyzed. Sodium azide was not detected in any of the samples.

Journal

Citations (1)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top