杜仲葉を主原料とした健康食品中のゲニポシド酸及びカフェインの分析

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タイトル別名
  • Analysis of Geniposidic Acid and Caffeine in Leaves of <i>Eucommia ulmoides</i> Used in Health Foods
  • トチュウハ オ シュゲンリョウ ト シタ ケンコウ ショクヒンチュウ ノ ゲ

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説明

An HPLC method was developed for the determination of geniposidic acid (GA) and caffeine (CF) in leaves of Eucommia ulmoides used in health foods. GA and CF in dried leaves were simultaneously extracted with water under reflux by heating. The extract was cleaned up with a C18 and ion-exchange type cartridge, then GA and CF were separated on an ODS column, with a UV detector (GA, 240nm; CF, 275nm). The recoveries of GA and CF added to samples were 94.2-94.7% and 90.4-96.7%, respectively. The determination limits of GA and CF in dried leaves were 10μg/g, and those in beverage samples were 1μg/g.<br>The GA and CF contents of 15 commercial samples were surveyed. The contents of GA in dried leaves of only Eucommia ulmoides, in mixtures of dried leaves of Eucommia ulmoides and others, and in beverage samples, were 290-1, 500, 160-1, 300 and 3.6-19μg/g, respectively, while CF was only detected in the mixtures with leaves of green tea or oolong tea, at 2, 100-9, 800μg/g.

収録刊行物

  • 食品衛生学雑誌

    食品衛生学雑誌 37 (6), 343-350_1, 1996

    公益社団法人 日本食品衛生学会

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