Studies on Precursors of Chloroform Formed in Soybean Sprouts by Sodium Hypochlorite Treatment

DOI JASI Web Site 3 Citations 18 References

Bibliographic Information

Other Title
  • 次亜塩素酸ナトリウム処理もやしから生成するクロロホルムの前駆物質に関する検討
  • ジアエンソサン ナトリウム ショリ モヤシ カラ セイセイスル クロロホルム

Search this article

Description

Gel filtration of soybean sprouts treated with sodium hypochlorite (NaClO) affords a fraction showing a high level of chloroform (CHCl3), and this was studied to identify the precursors of CHCl3.<br>Eight spots were observed on the plate in thin layer chromatography of the fraction, and their Rf values suggested that they might be amino acids. The mass spectral ion peaks of the fraction obtained by LC/MS suggested the presence of ten kinds of amino acids. The presence of these amino acids was confirmed by HPLC of the fraction, and among them, L-asparagine showed the highest value of 131.1μg/mL. When the ten kinds of amino acids thus detected were treated with NaClO, CHCl3 was formed from seven of them. An amino acid standard solution prepared with amounts similar to those in the fraction was treated with NaClO for 24 hours. The consequent CHCl3 formation was 94.2% of that formed from the fraction. Thus, the amino acid constituents identified in the gel filtration fraction are considered to be the precursors of CHCl3 formed in soybean sprouts by NaClO treatment.

Journal

Citations (3)*help

See more

References(18)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top