Analytical Method of Cereulide in Processed Cereal-Based Foods and Milk Powder by LC-MS/MS

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  • ＬＣ－ＭＳ／ＭＳによる穀物加工食品および粉ミルク中のセレウリドの分析法
  • LC MS MS ニ ヨル コクモツ カコウ ショクヒン オヨビ コナミルク チュウ ノ セレウリド ノ ブンセキホウ

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Abstract

An LC-MS/MS method for analysis of cereulide, an emetic toxin produced by Bacillus cereus, was developed. Cereulide was extracted from samples, fried rice, pan-fried noodles, red bean paste and baby formula, with methanol and purified using Oasis HLB cartridges. LC separation was performed on a C18 column with a mixture of formic acid solution and methanol containing ammonium formate as a mobile phase, and the mass spectrometer was operated in the positive electrospray ionization mode. Performance evaluation showed that trueness was higher than 70% and repeatability and reproducibility were within 10%. The limits of quantification were lower than 1 μg/kg.

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