Investigation to Find an Indicator Substance for the Analysis of Sodium Copper Chlorophyllin in Foods

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  • 食品中の銅クロロフィリンナトリウム分析における指標成分
  • ショクヒンチュウ ノ ドウ クロロフィリン ナトリウム ブンセキ ニ オケル

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A suitable indicator for the analysis of sodium copper chlorophyllin (Cu-Chl-Na) in foods was investigated by HPLC with a photodiode array detector. Cu-Chl-Na was extracted with ethyl ether from the homogenized sample after pH adjustment to 3-4 by adding 0.1N hydrochloride. The extracted solution was concentrated to dryness, then the residue was dissolved in methanol as the test solution. Measurement of Cu-Chl-Na was achieved on a Inertsil ODS-2 column with a mobile phase of methanol-H2O (97:3) containing 1% acetic acid, at a wavelength of 405nm.<br>Cu-Chl-Na on the market was a mixture that mainly consisted of copper chlorin e6 and copper chlorin e4, i. e., Cu-Chl-Na is not a single substance. In some cases, copper chlorin e6 disappeared, depending on the influence of pH and heat treatment during the manufacturing process of foods. On the other hand, copper chlorin e4 was relatively stable under these conditions. Therefore, copper chlorin e4 could be used as an indicator substance for the analysis of Cu-Chl-Na.<br>Cu-Chl-Na in boiled bracken, agar-agar and chewing gum was analyzed by using copper chlorin e4 as an indicator substance.

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