Protective Effects of Interleukin-10 on Diaphragm Muscle in a Septic Animal Model.

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  • Taneda Azusa
    The First Department of Internal Medicine, Tohoku University School of Medicine
  • Shindoh Chiyohiko
    The First Department of Internal Medicine, Tohoku University School of Medicine
  • Ohuchi Yuzuru
    The First Department of Internal Medicine, Tohoku University School of Medicine
  • Shirato Kunio
    The First Department of Internal Medicine, Tohoku University School of Medicine

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The effects of Interleukin (IL)-10 intravenous injection after endotoxin administration on diaphragm muscle were studied using Wistar rats. The animals were divided into two treatment groups: A saline+endotoxin group as control and an IL-10+endotoxin group. E. coli endotoxin (10 mg/kg) was injected intraperitoneally 5 minutes after saline or IL-10 (1250 U, 0.25 mg) injection. The force-frequency curves, twitch kinetics and fatigability were measured at 0 and 4 hours after endotoxin injection. In the saline+endotoxin group, the force-frequency curves and half relaxation time were significantly decreased at 4 hours (p<0.001 and p<0.05, respectively) compared to those at 0 hour. In the IL-10+endotoxin group, the decrement in the force-frequency curves by endotoxin was prevented at 4 hours compared to that at 0 hour. Nicotinamide adenine dinucleotide phosphate (NADPH) diaphorase histochemistry showed positive staining in the saline+endotoxin at 4 hours, but there was no significant staining at 0 or 4 hours in the IL-10+endotoxin group. These data suggest that IL-10 prevents the deterioration of contraction induced by endotoxin by inhibiting nitric oxide production in the diaphragm muscle.

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