The Kinetics of Glycerol and Dimethyl Sulfoxide in Cultured Leukemic and Burkitt Lymphoma Cells

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  • NIWAYAMA GEN
    Department of Pathology, School of Medicine, University of California, Los Angeles, California, and Tumor Procurement Laboratory, Roswell Park Memorial Institute

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This experiment was designed to study the kinetics of glycerol and dimethyl sulfoxide (DMSO) in cultured cells of human leukemia and lymphoma at 0°C and 37°C. Quantitative photographic measurement of changes of cell diameter was used as assay of relative permeability. Movement of DMSO was generally found to be more rapid than that of glycerol. Movement of both agents was found to be more rapid at 37°C than at 0°C. Changes in cell diameter was marked with glycerol than with DMSO. Equilibration time of DMSO, as assayed by change in cell diameter at 0°C was approximately 15 to 20 minutes as contrasted with 20-25 minutes for glycerol.

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