Glycans and glycoproteins in 0.05M LaCl3-soluble fraction from bovine costal cartilage.

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  • Glycans and Glycoproteins in 0 05M LaCl

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The supernatant fraction (Fr. S) obtained by ten-fold dilution of the 0.5M LaCl3-extract of bovine costal cartilage was fractionated by DEAE-Sephadex A-25 column chromatography, followed by gel filtration on Sepharose 4B. The resulting five subfractions were characterized by electrophoresis on cellulose acetate membranes before and after digestion with pronase, α-amylase, and chondroitinase AC, together with analytical data. The results showed that Fr. S contained a large quantity of glycogen (Fr. 1), three sialoglycoproteins (Frs. 2, 3 and 4-1), hyaluronic acid (Fr. 4-1), a small molecular size proteoglycan consisting of chondroitin sulfates and keratan sulfate (Fr. 4-2), and small quantities of collagen-like protein. The components in the correspond-ing subfractions of Fr. S from non-calcified (NCC) and calcified (CC) portions of the costal cartilage were similar. It is indicated that consumption of glycogen is remarkable on calcification.

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