Phytohemagglutinin-Induced Cytotoxic Action of Normal Lymphocytes on Cells in Tissue Culture Using <sup>14</sup>C-Leucine Incorporation

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  • Phytohemagglutinin-Induced Cytotoxic Action of Normal Lymphocytes on Cells in Tissue Culture Using 14C-Leucine Incorporation
  • Phytohemagglutinin Induced Cytotoxic Ac

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Abstract

Stimulation of normal peripheral blood lymphocytes (PBL) by phytohemagglutinin (PHA) and production of PHA-induced cytotoxicity were investigated. PBL transforma-tion stimulated by PHA was measured by 3H-thymidine incorporation, and the cytotoxicity was assayed by measuring the reduction of 14C-leucine incorpora-tion into target cells after interaction with PHA-treated PBL. The cytotoxicity of PHA-treated PBL showed dose response curves which corresponded with that of PBL transformation. Reduced 14C-Leucine incorporation of target cells caused by cytotoxic PBL would be a reliable measure of immune reactions.

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